S1B-5 Macrophage Gene Therapy in the Central Nervous System

S1B-5 Macrophage Gene Therapy in the Central

Nervous System

So Yoon Lee; Cara Orji; ZHANG Zi-jian; ZHANG Hao-yun; Anthony Bastian; Adriana Galvez; DOU Huan-yu

【期刊名称】《《神经药理学报》》 【年(卷),期】2018(008)004

【摘要】Regulation of immune responses is particular important in recovery after stroke,traumatic brain injury,and brain infections by inhibiting inflammation and reinstating rescue environment.Gene delivery based immunotherapy appears to have significant therapeutic potential for the treatment of neurological conditions by inhibition of neuroinflammation

to

prevent

irreversible

destructive

mechanisms.However,current gene delivery systems lack desired convenience,efficiency,and safety.Nanocarriers offers an attractive gene delivery system that overcome the immunogenic and long-term oncologic effects of the viral vectors.Using branched PEI lipids with PLGA,we developed a highly efficient core-shell nanoparticle(NP)gene delivery system.These IL-4 plasmid DNA(pIL-4)loaded NPs(pIL-4-NPs)were effectively delivered pIL-4 to the human blood monocytes derived

macrophages(MDM)and

mouse

bone

marrow-derived

macrophages(BMM).Furthermore,pIL-4-NPs quickly escaped lysosomes and entered the nuclei of these cells.In in vitro transfection assays,the