DEVELOPMENTAL STUDIES OF ELECTRICAL PROPERTIES IN CULTURED RAT BRAIN NEURONS

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作者：王海薇;吕国蔚;张炳烈;李文彬;;;;;

作者机构：Department of Neurobiology;Capital Institute of

Medicine;Beiling 100054;PRC;Institute of Basical Medicine;Academy of Military Medical Sciences;Beijing 100850;PRC; 来源：中国科学年：1990 卷：000 期：001 页码：P.35-43 页数：9 中图分类：O6 正文语种：CHI

关键词：cultured neurons development membrane electrical proper ties ionic channel blockers.

摘要：The rat brain neurons isolated from 15—17-day embryos were grown in dissociated cell culture and maintained in vitro for 3 weeks, The developmental process of the neurons was observed from the early stage of development in terms of passive and active electrical parameters and their changes induced by perfusion of ionic channel blockers.The neurons’ development could be morphologically divided into stages Ⅰ (from seedling to 5 days), Ⅱ (7—11 days), Ⅲ (12—20 days)andlV (21—22 days). The relative values of resting membrane potentials (RMP) for stages Ⅰ, Ⅱ, Ⅲ and Ⅳ were 1, 3.46, 4.07 and 6.54, respectively; the corresponding values of input resistance (Rin) were 10

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