HPLC法测定复方雷公藤糖浆中雷公藤甲素的含量

HPLC法测定复方雷公藤糖浆中雷公藤甲素的含量

成西霞;李云霞

【期刊名称】《中国药师》 【年(卷),期】2014(000)002

【摘要】Objective:To establish a method for the content determination of triptolide in compound tripterygium wilfordil syrups by HPLC. Methods:The samples were analyzed on a Diamonsil C18 column(150 mm × 4. 6 mm,5μm), the mobile phase consisted of methanol and water (46∶54), the flow rate was 1. 0 ml·min-1, the detection wavelength was 224 nm, and the column temperature was kept at 30℃, and the injection volume was 10 μl. Results: The linear range of triptolide was 5. 66-33. 96 μg · ml-1 ( r =0. 999 8), and the average recovery was 99. 35% with RSD of 1. 02%(n=9). Conclusion:The method is simple and sensitive with good repeatability, and can be used to control the quality of triptolide in compound tripterygium wilfordil syrups.%目的：：建立高效液相色谱法测定复方雷公藤糖浆中雷公藤甲素的方法。方法：采用 Diamonsil C18(150 mm ×4.6 mm,5μm)色谱柱分离,甲醇-水(46∶54)为流动相,流速为1.0 ml·min-1,检测波长为224 nm,柱温为30℃,进样量为10μl。结果：雷公藤甲素在5.66~33.96μg ·ml-1(r=0.9998)范围内质量浓度与峰面积呈良好的线性关系,其平均回收率为99.35%(RSD=1.02%,n=9)。结论：本方法简便、灵敏、重复性好,可用于复方雷公藤糖浆中雷公藤甲素的质量控制。