RNAi技术沉默STAT1基因对哮喘小鼠气道炎症的影响

RNAi技术沉默STAT1基因对哮喘小鼠气道炎症的影响

刘春凤;任磊;熊瑛

【期刊名称】《泸州医学院学报》 【年(卷),期】2015(000)001

【摘要】Objective: To discuss the effects of RNA interference (RNAi) silencing the gene of signal transducer and activator of transcription 1(STAT1) on airway inflammation of bronchial asthmatic mice. Methods:4 weeks old female BALB/c from 32 mice were randomly divided into normal group, PBS group, empty plasmid ( random fragment RNAi ) group and siRNA (pGSTAT1-2) group. RNAi was used to silence the gene of STAT1 of bronchial asthmatic mice. S-P method was used to detect the expression of STAT1 and ELISA was used to detect the concentration of airway inflammation medium such as IFN-γ and IL-5 from the bronchoalveolar lavage fluid (BALF) by the single lung lavage. Results: The concentration of IFN-γ of siRNA group was higher than that of PBS group and empty plasmid group (P<0.05), but the concentration of IL-5 was opposite (P<0.05). There was no significant difference between PBS group and empty plasmid group (P 跃 0.05). The count of EOS and the concentration of IL-5 were significantly decreased in empty plasmid group and the PBS group, but the concentration of IFN-γ increased, indicating negative correlation between IFN-γ and EOS, and positively correlation between IL-5 and