Transcriptome analysis of sugar beet root maggot (Tetanops myopaeformis) genes modulated by the
Beta vulgaris host
Haiyan Li;Ann C.Smigocki
【期刊名称】《中国昆虫科学：英文版》 【年(卷),期】2018(025)002
【摘要】Sugar beet root maggot (SBRM,Tetanops myopaeformis von R(o)der) is a major but poorly understood insect pest of sugar beet (Beta vulgaris L.).The molecular mechanisms underlying plant defense responses are well documented,however,little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance.To date,no studies have been published on SBRM gene expression profiling.Suppressive subtractive hybridization (SSH) generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant (F 1016) and a susceptible (F1010) sugar beet line.Blast2GO v.3.2 search indicated that over 40％ of the differentially expressed genes had known functions,primarily driven by fruit fly D.melanogaster genes.Expression patterns of 18 selected EST clones were confirmed by RT-PCR analysis.Gene Ontology (GO) analysis predicted a dominance of metabolic and catalytic genes involved in the interaction of SBRM with its host.SBRM gencs functioning during development,regulation,cellular
相关推荐：
loading