三号黑体居中 摘 要 小四号宋体 内蒙古白绒山羊是一种优良家畜品种，通过EST（表达序列标签）测序在分子水平上获得大量数据，这些数据提供了内蒙古白绒山羊的部分基因信息，为进一步研究与山羊绒生长相关基因奠定基础。
本实验选择内蒙古白绒山羊毛囊兴盛期cDNA文库为研究材料，其中15条HGT （高甘氨酸/酪氨酸角蛋白关联蛋白）的cDNA序列已被国际公共数据库—GenBank数据库接受，其中KRTAP6（角蛋白关联蛋白家族6）家族成员6个，KRTAP7家族成员1个，KRTAP8家族成员2个，KRTAP16成员6个。
通过对不同物种HGT氨基酸序列的比较分析，发现山羊HGT的氨基酸序列与其它哺乳动物如人、小鼠和绵羊的HGT氨基酸序列有很多一致或保守的区域。分析其蛋白功能位点，发现在山羊的HGT中有许多豆蔻酰化位点。
通过对白绒山羊HGT蛋白的二级结构预测，发现绒山羊HGT和其它物种HGT的二级结构中无规则线性长链结构所占比例最多，β-伸展结构占到了较少的部分，几乎没有发现α-螺旋的存在。
小四号黑体
关键词：绒山羊；生物信息学；表达序列标签；高甘氨酸/酪氨酸角蛋白关联蛋白（HGT）
小四号宋体
三号Times New Roman，加粗、居中 Abstract
小四号 Times New Roman
In this study，15 HGT cDNA sequences were submitted to the international public database (GenBank) which were chosen from the cDNA library of the Inner Mongolia white cashmere goat during anagen with skin as study material. Among them we find 6 members in KRTAP 6, 1 members in KRTAP7, 2 members in KRTAP8 and 6 members of KRTAP16.
Compared the amino acids sequence of HGT of different species, the results showed that the HGT amino acids sequence of goat had many conservative or identical domain with Human, Mouse and Sheep. Analyzing its Motif discovers that goat HGT have many N-myristoylation site.
The secondary structure of HGT was predicted, the result indicated irregular long chain had a large proportion in the HGT secondary structure of goat and other species, there were a small quantity of β- extended structures and α- helix were almost not found.
四号Times New Roman 加粗
小四号 Times New Roman Key Words：Cashmere Goat；Bioinformatics；EST(Express Sequence Tags)；
HGT(high glycine/ tyrosine Keratin Associate Protein KRTAP)
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