S2B-5 Rapid Release of GABA Inhibiting Epilepsy in Vivo

S2B-5 Rapid Release of GABA Inhibiting Epilepsy in

Vivo

DING Ran; LI Yuan-yuan; QIAO Zhi-hong; LIU Nan-nan; ZHANG Xin; WEI Liang-peng; HOU Meng-zhu; LIU Ai-li; ZHENG Chen; FU Bing; XU Ying-shun; MA Zheng-guan; HOU Shao-wei; WANG Fei-fei; YUAN Shi-yang; LIU Shu-jing; ZHANG Yan; SHEN Hui

【期刊名称】《《神经药理学报》》 【年(卷),期】2018(008)004

【摘要】Objective:Epilepsy is a multi-etiological brain dysfunction syndrome characterized by synchronously repeated spontaneous discharges

from

neuronal

cells.Its

pathogenesis

involves

excitatory/inhibitory imbalance,ion channel abnormalities and may be related to the abnormal structure and function of neurotransmitter receptors.The research was to observe the effects of rapid increase inhbitory

neurotransmitter

GABA

on

the

excitatory/inhibitory

imbalance,ion channel abnormalities and abnormal neurotransmitter receptors in living epileptic mice from the level of free moving animal,cell,sub-cell and receptor.Methods:The techniques of fiber photometry and laser uncage of Rub-GABA for epileptic free moving mice induced by administration of Kainic acid(KA)or 4-AP,local field potentials(LFP),multicell bolus lording of calcium(OGB-1AM),virus transfection of calcium-sensitive protein GCaMP6f,transfection of fluorescent labeled AMPA receptors in cortical neurons by in utero