晋升推荐医生职称晋升所需常见呼吸类优秀论文 (41)

晋升推荐医生职称晋升所需常见呼吸类优秀论文

原发性支气管肺癌中PTEN基因的蛋白表达及意义

作者：李雅莉，辛丽红，杨侠，张洁，李家芝，刘云 作者单位：西安交通大学医学院第二附属医院呼吸内科，陕西西安 710004

【摘要】 目的 研究抑癌基因PTEN在原发性肺癌中的蛋白表达及其临床意义。方法 应用免疫组织化学SP法检测56例手术切除的肺癌石蜡标本及3例正常肺组织中的PTEN基因的蛋白表达情况。结果 ①正常肺组织中PTEN蛋白表达的阳性率明显高于肺癌患者，而在小细胞肺癌中的阳性表达低于非小细胞肺癌；②在56例肺癌患者中，随着分化程度的降低，PTEN的蛋白阳性表达率也随之下降；③Ⅰ、Ⅱ期肺癌患者的PTEN蛋白阳性表达率高于Ⅲ、Ⅳ期患者；④在19例有转移的肺癌患者中，PTEN表达阳性率明显低于无转移的患者。结论 PTEN基因的表达与肺癌的病理类型、组织的分化程度、临床分期以及有无淋巴结转移密切相关。

【关键词】 肺癌；PTEN基因；免疫组织化学

Expression of PTEN protein in primary lung cancer

Li Yali, Xin Lihong, Yang Xia, Zhang Jie, Li Jiazhi, Liu Yun

(1. Department of Respiration, the Second Affiliated Hospital,Medical School of Xi’an Jiaotong University, Xi’an 710004;2. Department of Respiration, Xi’an Children’s Hospital, Xi’an 710002, China)

ABSTRACT: Objective To study the protein expression level and clinicalsignificance of the tumor suppressor gene PTEN in primary lung cancer. Methods We detected the protein expression of PTEN gene in paraffin specimens of 56 cases of lung cancer and 3 specimens of normal lung tissues by SPimmunohistochemistry. Results ① The positive rate of PTEN protein expression was higher in normal lung tissues than in lung cancer specimens, while it was lower in SCLC than in NSCLC. ② The positive rate of expression of PTEN protein became lower as the differentiation level decreased in the 56 cases of lung cancer specimens. ③ The positive rate of expression of PTEN protein was higher in patients with lung cancer of stage Ⅰ and Ⅱ than that in stage Ⅲ and Ⅳ. ④ The positive rate of expression of PTEN protein was 26.32% in 19 cases of transfused specimens, which was obviously lower than that in non-transfused specimens. Conclusion The expression of PTEN gene is related to classification criteria of lung cancer, malignancy degree, clinical stage and lymph node transfusion.

KEY WORDS: lung cancer; phosphatase and tensin homologue deleted from chromosome 10 gene(PTEN); immunohistochemistry

肿瘤的发生是多阶段、多步骤和多基因改变的过程，肿瘤的恶性表型是多种因素相互作用导致正常细胞恶变的结果，而癌基因的激活和抑癌基因的失活在肿瘤的发生发展中起重要作用。对抑癌基因的研究不仅在探索肿瘤发生的分子生物学机制方面有重要意义，而且在肿瘤的预防、治疗、诱导分化、凋亡、衰老等方面也有重大价值。PTEN基因(phosphatase and tensin homologue deleted from chromosome 10 gene)又称MMAC1(mutated in multiple advanced cancers gene)或TEP1(TGF-regulated and epithelial phosphatase 1 gene)，是1997年由Steck等［1］3个研究小组分别发现并命名的一种新的抑癌基因(简称PTEN)。现已证实该基因的突变、失活与多种肿瘤的发生发展密切相关。为探讨原发性支气管肺癌中PTEN基因的蛋白表达与肺癌发生发展的关系，我们用免疫组织化学方法对56例肺癌患者石蜡标本及3例正常肺组织标本进行了研究。

1 材料与方法

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