S1B-4 3D Genome Mapping in Dopaminergic Neurons from Adult Human Midbrain

S1B-4 3D Genome Mapping in Dopaminergic

Neurons from Adult Human Midbrain

Gill SE; Halene T; Rajarajan P; Chandrasekaran S; Kassim B; Fullard JF; Loh YHE; Bannon MJ7; Crawford GE; Roussos P; Ossowski S; Tsankova NM; JIANG Yan

【期刊名称】《《神经药理学报》》 【年(卷),期】2018(008)004

【摘要】Objective:Monoamine system plays a key role in the pathophysiology of a wide range of neuropsychiatric disorders including Parkinson’s,Huntington’s,drug addiction and many other conditions.Of note,there is a large amount of literatures exploring monoamine pathways in the context of adaptive mechanisms inside the cell nucleus,including changes in gene expression and alterations in chromatin modifications,structure and functions.However,such types of studies were largely focused on forebrain structures that receive input from monoaminergic neurons.Comprehensive genome-scale and cell-type specific mappings is lacking for brainstem areas that consist of monoaminergic neurons,including the substantia nigra(SN)and ventral tegmental area(VTA).This is especially the case for human study,due to the limited access to clinical human brain samples and lack of efficient assay for cell-type specific isolation from postmortem brain tissues after low-temperature or fixative preservations.Here,we introduce novel protocol to comprehensively map the 3D genome and nuclear