HPLC-荧光法同时测定人血浆中氯沙坦及其活性代谢物E-3174的浓度

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杨璐;夏东亚;郭涛

【期刊名称】《中国药房》【年(卷),期】2009(020)032

【摘要】To develop an HPLC-fluorescence method for simultaneous determination of losartan and its major metabolite(E-3174) in human plasma. METHODS. Plasma sample was pretreated by liquid-liquid extraction with aether then determined with valsartan served as internal standard. The determination was performed on Diamonsil C_(18) with column temperature set at room temperature. The mobile phase consisted of 0.02 mol·L~(-1) sodium dihydrogen phosphate buffer (adjusted to pH=2.35 with phosphoric acid) - acetonitrile (57 : 43) at a flowrate of 0.5 mL·min~(-1). The excitation wavelength was set at 250 nm and the emission wavelength was set at 370 nm. RESULTS: The linear range of losartan was 10～1 000 ng·mL~(-1) (r = 0.999 0) with a lowest limiest of quantification(LLOQ) of 10 ng·mL~(-1); the linear range of E-3174 was 5～1 000 ng·mL~(-1) (r = 0.999 0) with a LLOQ of 5 ng·mL~(-1). The methodological recoveries of losartan and E-3174 were 94.05%～110.09% and 107.7%～110.94%, respectively, with both intra-day RSD and inter-day RSD at less than 10.0%; the extraction recoveries of losartan and E-3174 were 69.16%～70.85% and 67.50%～70.77% ,

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