OsBTF3基因在水稻光合作用和生长发育中的功能

OsBTF3基因在水稻光合作用和生长发育中的功能

李广旭;陈华民;吴茂森;何晨阳

【期刊名称】《作物学报》 【年(卷),期】2012(038)005

【摘要】为了揭示转录因子OsBTF3在水稻生长发育中的功能,比较分析了OsBTF3过量表达和RNAi转基因水稻T1代株系叶片生长和株型发育的表型.结果表明,与野生型对照株相比,过量表达株系在叶绿素含量、叶绿体数量、光合速率、叶片大小、株高、节间长方面显著增加或增强；而RNAi株系的明显降低或减弱.OsBTF3基因的增量或减量表达显著影响水稻光合作用、叶片生长和株型发育.说明OsBTF3在水稻生长发育中具有重要调控功能,可能在水稻转基因分子育种方面具有潜在的应用价值.%To reveal the regulatory roles of transcription factor OsBTF3 in growth and development of rice, we comparably analyzed the growth and development of the transgenic rice with over-expressed or RNAi-silenced OsBTF3 gene. Results showed that significant increases in chlorophyll content, chloroplast number, photosynthetic rate, leaf size, plant heights and stem inter-node length were found in T, generation of transgenic rice lines with the over-expressed gene compared to wild-type control plants, while remarkable decreases in above phenotypes were observed in plants with the RNAi-silenced gene. Over-expression or silencing of transgene OsBTF3 in rice resulted in significant changes in photosynthesis, growth and development of rice. Therefore, OsBTF3 functions as a key regulator in