两种检测超广谱β-内酰胺酶表型方法的比较

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严育忠;范惠清;徐英;周秀梅;陆燕春

【期刊名称】《中国感染控制杂志》【年(卷),期】2012(011)005

【摘要】Objective To investigate antimicrobial resistance and genotypes of extended-spectrum β-lactamases (ESBLs)-producing strains isolated from clinic in a hospital, and to establish an effective method to analyze ESBL phenotypes. Methods One hundred ESBL positive isolates (initial screen test was positive) were randomly selected as experimental group, and 30 ESBL negative isolates (initial screen test was negative) was as control group. All strains were identified by Vitek-2 Compact automatic system, and antimicrobial susceptibility test was performed by Kirby-Bauer disc diffusion method; Carbapenem-resistant isolates were detected by modified Hodge test; β-lacta-mase phenotypes were detected by both ESBL phenotypic confirmatory test recommended by Clinical Laboratory Standard Institute (CLSI) and modified ESBL confirmatory test incorporating 3-aminophenylboronic acid; ESBL genes of all isolates were detected by PCR and DNA sequencing. Results ESBL screen positive isolates were all resistant to cefotaxime, but susceptible to carbapenems; the modified Hodge tests were positive in two carbapenem-resistant isolates. The detection rate of ESBL genes was 84. 00% , the dominant genes were SHV and CTX-M type. PCR detection for

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