HPLC测定白花泡桐叶不同月份熊果酸和木犀草素的含量

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吾鲁木汗·那孜尔别克;石进校;李科;姚福成;恩特马克·布拉提白

【期刊名称】《湖南农业科学》【年(卷),期】2011(000)021

【摘要】为了比较湘西宏成制药有限责任公司不同中药种子繁殖基地白花泡桐叶中熊果酸和木犀草素6月到9月间含量的变化,采用Shim-Pack C18色谱柱,测定熊果酸的流动相为甲醇-水-乙酸(85:15:0.5),检测波长210 nm;测定木犀草素的流动相为甲醇-水-乙酸(60:39:1),检测波长350 nm.结果显示,熊果酸的线性范围为1～15μg(r=0.999 6),平均回收率为98.3％(RSD=1.10％,n=5)；木犀草素的线性范围为0.01～0.09 μg(r=0.999 1),平均回收率为98.7％( RSD=2.25％,n=5)；研究结论为7月份的白花泡桐叶中熊果酸和木犀草素含量最高,为药物原料的采收时间的划定提供了理论依据.%The changes of ursolic acid content and luteolin content in leaves of Paulownia fortune, which produced from different propagation bases of Xiangxi Hongcheng Pharmaceutical Co., Ltd. In Western Hunan, from June to September were compared. The content of ursolic acid and luteolin was determined by HPLC, respectively, and the analytical column was Shim-Pack C18; for detecting content of ursolic acid, the mobile phase consisted of methanol-water-acetic acid (85:15:0.5) at a flow rate of 1.0 mL/min, and the detection wavelength at 210 nm; for detecting content of luteolin, the mobile phase consisted of methanol-water-acetic acid (60:39:1) at a flow rate of 1.0 mL/min, and the detection wavelength at

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