HPLC测定葛灵胶囊中葛根素的含量
雷德卿;李帅;刘银凤
【期刊名称】《食品与药品》 【年(卷),期】2014(000)001
【摘要】Objective To establish the method for determination of puerarin in Geling Capsules by HPLC. Methods Analytical column was Golden C18, the mobile phase consisted of 0.1 mol/L acetic acid-methanol(75:25), the flow rate was 1.0 mL/min, the detection wavelength was 247 nm, and column temperature was 35℃. Results The peak of puerarin was symmetry and completely separated from other ingredients. The linear range of puerarin was 52.51-525.12 ng(r=1.0000, n=6), the average recovery was 99.7%,with RSD of 0.86%(n=9). Conclusion This method is accurate, simple and reliable. It can be used for the quality control of Geling Capsules.%目的：建立高效液相色谱法（HPLC）测定葛灵胶囊中葛根素含量。方法采用Golden C18（250 mm×4.6 mm，5μm）色谱柱，流动相为0.1 mol/L醋酸-甲醇（75：25），流速1.0 mL/min，检测波长247 nm，柱温35℃。结果葛根素与其他成分完全分离，峰形良好，在52.51~525.12 ng范围内线性关系良好（r=1.0000，n=6），平均加样回收率为99.7%，RSD为0.86%（n=9）。结论所建立的方法简便、准确、重现性好，可用于该制剂的质量控制。 【总页数】2页(46-47)
【关键词】高效液相色谱法;葛灵胶囊;葛根素;含量测定
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