柱前衍生化HPLC法检测紫苏子和紫苏叶中氨基酸的含量

柱前衍生化HPLC法检测紫苏子和紫苏叶中氨基酸的含量

祝洪艳;张荻;张力娜;李兵臣;赵岩;何忠梅;郜玉钢;张连学

【期刊名称】《药物分析杂志》 【年(卷),期】2017(037)010

【摘要】目的:研究紫苏子和紫苏叶中游离和水解氨基酸的种类及含量.方法:采用柱前衍生化高效液相色谱法检测,以异硫氰酸苯酯为衍生试剂,使用Ultimate Amino Acid氨基酸分析柱(4.6 mm×250 mm,5μm),流动相A为乙腈-水(80∶20),流动相B为醋酸钠缓冲液(pH 6.5)-乙腈(93∶7),流速1.0 mL·min-1,检测波长254 nm.结果:21种氨基酸均呈现良好的线性关系(R2＞0.999 0),紫苏子和紫苏叶中水解氨基酸的平均回收率分别为93.21％～101.6％(RSD为0.87％～2.9％,n=6)和96.88％～100.8％(RSD为0.68％～2.7％,n=6).紫苏子中检测到17种游离氨基酸,含8种人体必需氨基酸;紫苏叶中检测到18种游离氨基酸,含7种人体必需氨基酸,且紫苏叶中氨基酸含量高于紫苏子.结论:本法可用于紫苏中氨基酸的含量测定.%Objective:To investigate the varieties and contents of free and hydrolyzed amino acids in Perillae Fructus and Perillae Folium.Methods:HPLC with pre-column derivatization was employed,with

phenyl

isothiocyanate

(PITC)

as

derivatization

reagent.Separation was performed on an Ultimate Amino Acid specified column (4.6 mm × 250 mm,5 μm) and the mobile phase consisted of acetonitrile-water (80 ∶ 20) and sodium acetate (pH 6.5)-acetonitrile (93 ∶ 7) at a flow rate of 1.0 mL· min-1.The detection wavelength was set at 254 nm.Results:The calibration curves of 21 kinds of amino acids

showed good linearity within their test ranges.And the average recovery rates of the hydrolyzed amino acids in Perillae Fructus and Perillae Folium ranged from 93.21％ to 101.6％ (RSD 0.87％-2.9％,n=6) and 96.88％-100.8％ (RSD 0.68％-2.7％,n=6),respectively.17 Kinds of amino acid were detected in Perillae Fructus,8 of which were essential amino acid.18 Kinds of amino acid were detected in Perillae Folium,7 of which were essential amino acid.The amino acid contents in Perillae Folium were higher than those in Perillae Fructus.Conclusion:The method could be used in the determination of amino acids in Perillafrutescens (L.) Britt. 【总页数】7页(1858-1864)

【关键词】紫苏;紫苏子;紫苏叶;氨基酸;异硫氰酸苯酯;PITC;衍生试剂;高效液相色谱

【作者】祝洪艳;张荻;张力娜;李兵臣;赵岩;何忠梅;郜玉钢;张连学

【作者单位】吉林农业大学中药材学院,长春130118;吉林农业大学中药材学院,长春130118;吉林农业大学中药材学院,长春130118;吉林省安图县安图林业有限公司,安图133600;吉林农业大学中药材学院,长春130118;吉林农业大学中药材学院,长春130118;吉林农业大学中药材学院,长春130118;吉林农业大学中药材学院,长春130118 【正文语种】中文 【中图分类】R917 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-

journal-pharmaceutical-analysis_thesis/0201241244710.html 【相关文献】

1.DTDPA-PITC联合柱前衍生化HPLC法同时测定复方氨基酸注液中18种氨基酸的含量 [J], 阚微娜; 滕艳坤; 杨宏伟; 隗笑

2.柱前衍生化HPLC法同时测定阿胶中4种主要氨基酸的含量 [J], 程显隆; 肖新月; 邹秦文; 李广华; 田守生

3.百令胶囊中17种氨基酸的柱前衍生化RP-HPLC法含量测定 [J], 邹秦文; 肖新月; 程显隆; 刘燕; 张昀; 林瑞超

4.雷丸中16种氨基酸的柱前衍生化RP-HPLC法含量测定 [J], 郑灏; 程显隆; 魏锋; 肖新月; 林玉莲

5.柱前衍生化RP-HPLC法测定中药全蝎中16种氨基酸含量 [J], 梁琨; 安叡; 尤丽莎; 王新宏; 王峥涛

以上内容为文献基本信息，获取文献全文请下载