双波长比例法测定单细胞活性氧探析

双波长比例法测定单细胞活性氧探析

赵成瑞;崔香丽;吴博威

【期刊名称】《山西医科大学学报》 【年(卷),期】2011(042)001

【摘要】Objective To establish the double wavelengths ratio for detecting the concentrations of reactive oxygen species( ROS) in single cell. Methods ①Adult rat cardiomyocytes were isolated by enzymatic dissociation. Fluorescence signals of the single cell were recorded by the ion imaging system for finding Lhe two excitation wavelengths which made the biggest and the smallest fluorescence signal intensities. ②Cardiomyocytes

were

perfused

with

BPS4

to

imitate

the

ischemia/reperfusion model. Some cardiomyocytes were reperfused with

GSH.

The

intracellular

ROS

concentrations

were

measured. ,Results ①The values of the fluorescence intensity at 480 nm and 420 nm were the higgest and smallest . respectively. F480/F420 was used to represenl the intracellular ROS concentrations. ②At mimic ischemia and reperfusion stages,intracellular ROS fluorescence intensities ( F480/F420) increased progressively to ( 115. 27 ±4. 52) ％ and ( 1 16. 99 ± 3. 99 ) ％ of pre-ischemia respectively. When CSH was used in reperfusion , ROS fluorescence intensity decreased quickly to ( 101. 14 ±3. 20) ％ of pre-ischemia. Conclusion Double wavelengths ratio could be used to detect intracellular ROS.%目的 建立双波长比例法测