HPLC法测定卡波姆类口腔黏附片中盐酸利多卡因的含量

HPLC法测定卡波姆类口腔黏附片中盐酸利多卡因的含量

符旭东;陈晨;李银科;王珊珊;谢向阳

【期刊名称】《中国药师》 【年(卷),期】2015(000)004

【摘要】目的：建立卡波姆类口腔黏附片中盐酸利多卡因的HPLC含量测定方法。方法：通过氯化钙沉淀供试品中的卡波姆，采用Welch C18色谱柱（250 mm ×4.6 mm，5μm），流动相为醋酸钠溶液（取冰醋酸50 ml，加水930 ml，用1 mol·l-1氢氧化钠调pH至3.40）-乙腈（70：30）；流速1.0 ml·min-1，检测波长254 nm，柱温40℃，进样量20μl。结果：在0.10~2.00 mg· ml-1的范围内，盐酸利多卡因的浓度与峰面积呈良好的线性关系（r=0.9999），平均回收率为100.2%，RSD为1.4%（n=9）。结论：本方法简单、准确、重复性好，可用于口腔黏附片中盐酸利多卡因的含量测定。%Objective:To establish a method to determine the content of lidocaine hydrochloride in buccal adhesive tablets contai-ning carbomer as an excipient by HPLC. Methods:Calcium chloride was used to precipitate carbomer in buccal adhesive tablets. The HPLC analysis was performed on a Welch C18 column(250 mm × 4. 6 mm,5 μm)with the column temperature of 40℃. The mobile phase was the mixture of 0. 05% sodium acetate solution(added 50 ml acetic acid into 930 ml distilled water,adjusting pH to

3.

40

with 1

ml·min-1

sodium

hydroxide

solution)and

acetonitrile(70:30). The flow rate was 1. 0 ml·min-1 ,the detection wavelength was at 254 nm and the injection volume was 20 μl.