益肾固本法对慢性HBV携带者外周血干扰素调节因子-4
表达的影响
林巧;刘腊香;张国良;邓欣;刘晋洪;何清;聂广
【期刊名称】《中西医结合肝病杂志》 【年(卷),期】2013(023)003
【摘要】目的:从mRNA和蛋白水平两个层面探讨益肾固本方对慢性HBV携带者外周血IRF(干扰素调节因子)-4表达的影响.方法:选取慢性HBV携带者46例,其中27例服用益肾固本方进行中药干预,19例作为对照组不予任何药物治疗,动态随访24周后,采集外周血分离外周血单个核细胞(PBMC),利用流式细胞技术(FACS)观测CD4+T细胞中IRF-4+细胞比例,利用实时定量PCR(聚合酶链反应)技术检测两组人群IRF-4 mRNA水平,比较其IRF-4表达差异.结果:FACS可以有效鉴定IRF-4+细胞群,中药组IRF-4+细胞群峰值偏移程度小于对照组；中药组和对照组IRF-4+细胞比例分别为(2.61±0.19)％、(3.29±0.18)％,差异具有统计学意义(t=2.48,P=0.02)；中药组IRF-4 mRNA表达量显著低于对照组[(6.78±0.89) vs (10.66±1.18),t=2.67,P=0.01].结论:益肾固本方可以有效降低慢性HBV携带者IRF-4表达水平,对于纠正Thl/Th2细胞失衡、打破免疫耐受具有重要意义.%Objective:To explore the effect of Yishen Guben recipe on IRF-4 gene mRNA and protein expression in chro nic HBV carriers.Methods:Fourty-six chronic HBV patients were divided into 2 groups,involving 27 cases treated with Yishen Guben recipe,and 19 cases considered as control group without any treatment.After 24w dynamic follow-up,PBMC was isolated and IRF + cell population was
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