粪便中MGMT，p16INK4A及ECAD基因启动子甲基化
检测在结直肠癌诊断中的价值
冯凯瑜;范明娟;谭荣荣;沈波;史薇;曾峥
【期刊名称】《海南医学》 【年(卷),期】2016(027)021
【摘要】Objective To investigate the clinical value of MGMT, p16INK4A and ECAD gene promoter methylation in the diagnosis of colorectal cancer. Methods Fifty healthy people of more than 65 years old (control group), 41 patients of benign colorectal disease (benign colorectal disease group) and 46 patients with colorectal cancer (colorectal cancer group) in Department of Geriatrics from February 2014 to November 2015 were included in this study. The methylation levels of p16INK4A, MGMT, ECAD gene promoter in the stool of the three groups were assayed by methylation specific PCR. The diagnostic value of the p16INK4A, MGMT and ECAD methylation were calculated according to the Baye's equation. Results The methylation rates were 14.0%, 24.4%and 71.7%for p16INK4A gene in the three groups, 16.0%, 14.6% and 54.3% for MGMT gene, and 12.0%, 19.5% and 65.2% for ECAD gene respectively, with statistically significant difference between colorectal cancer group and benign colorectal disease group, control group (P<0.05). The diagnostic sensitivity was 0.72, 0.54 and 0.65, respectively, and the specificity was 0.76, 0.75 and 0.80 for p16INK4A, MGMT and
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