补骨脂乙素作用机制- Medchemexpress- MCE中国

Product Data?SheetIsobavachalconeCat. No.:CAS No.:分?式:分?量:作?靶点:作?通路:HY-1306520784-50-3C??H??O?324.37Akt; Reactive Oxygen Species; Apoptosis; AutophagyPI3K/Akt/mTOR; Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Apoptosis; Autophagy储存?式:4°C, protect from light* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)Inhibitors?Agonists?Screening Libraries溶解性数据体外实验DMSO : 11 mg/mL (33.91 mM; Need ultrasonic and warming)Mass1 mg5 mg10 mgSolventConcentration制备储备液1 mM5 mM10 mM3.0829 mL0.6166 mL0.3083 mL15.4145 mL3.0829 mL1.5414 mL30.8290 mL6.1658 mL3.0829 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；?旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存?式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个?内使?，-20°C 储存时，请在 1 个?内使?。BIOLOGICAL ACTIVITY?物活性Isobavachalcone (Corylifolinin) 来源于补?脂，是?种 Akt 信号通路的有效抑制剂，可诱导?类癌细胞凋亡 (以 IC50 值为 7.92 μM 抑制 OVCAR-8 癌细胞?长)，具有抗癌和抗增殖活性。Isobavachalcone 还能诱导 OVCAR-8 细胞中活性氧的产?。IC?? & Target体外研究IC50: 7.92 μM (OVCAR-8 cell)[1]Isobavachalcone (6.0-48.0 μM; 72 hours; OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells) treatment inhibits the proliferation of human cancer cells. Isobavachalcone inhibits PC3, A549, MCF-7, L-02 and HUVEC cells growth with IC50 values of 15.06 μM, 32.2 μM, 28.29 μM, 31.61 μM and 31.3 μM, respectively[1]. Isobavachalcone (0-18 μM; 6 hours; OVCAR-8 and PC3 cells) treatment results in a concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt and GSK3b phosphorylation[1]. Isobavachalcone (0-18 μM; 72 hours; OVCAR-8 and PC3 cells) treatment causes apoptosis via caspase- and ROS-involved mitochondrial pathway[1]. Page 1 of 2

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Cell Proliferation Assay[1]Cell Line:Concentration:Incubation Time:Result:Western Blot Analysis[1]Cell Line:Concentration:Incubation Time:Result:OVCAR-8 or PC3 cells0 μM, 6 μM, 12 μM, and 18 μM6 hoursA concentration-and time-dependent down-regulation of the Ser-473 phosphorylation of Akt. GSK3b phosphorylation was also inhibited in a concentration- and time-dependent manner.Apoptosis Analysis[1]Cell Line:Concentration:Incubation Time:Result:OVCAR-8 cells and PC3 cells0 μM, 6 μM, 12 μM, and 18 μM72 hoursLed to dose dependent increase of apoptosis.OVCAR-8, PC3, A549, MCF-7, L-02 and HUVEC cells6.0-48.0 μM72 hoursInhibited the proliferation of human cancer cells.体内研究Isobavachalcone (20 mg/kg; intraperitoneal injection; for 0.5-24 hours; female Kunming mice) treatment results in an increase in blood glucose levels, reaching a maximum within 1 hour and maintaining until 4 hours post-dosing[1].Animal Model:Dosage:Administration:Result:Seven-week-old specific pathogen-free female Kunming mice (18-22 g)[1]20 mg/kgIntraperitoneal injection; for 0.5, 1, 2, 4, 6, 8, 12, 24 hoursIncreased in blood glucose levels.客户使?本产品发表的科研?献????Int Immunopharmacol. 2013 Jan;15(1):38-41.See more customer validations on www.MedChemExpress.cnREFERENCES[1].?Jing H, et al. Abrogation of Akt signaling by Isobavachalcone contributes to its anti-proliferative effects towards human cancer cells. Cancer Lett. 2010 Page 2 of 3 www.MedChemExpress.cn

Aug 28;294(2):167-77.

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