医药,医学,内科学
A组链球菌壁多糖的抗原性及交叉反应性研究
2010-10-06 彭朝权 余步云 陈国庆 梁慧
【摘 要】 目的 研究A组β溶血性链球菌壁多糖的最具抗原性的部分，及与牛心瓣膜糖蛋白的交叉反应性。方法 设计了新的提取和纯化链球菌壁多糖抗原的技术—分子筛凝胶层析法，以双向琼脂免疫扩散法鉴定出最具抗原性的部分，并测定这部分多糖与牛心瓣膜糖蛋白交叉反应性。结果 发现A组链球菌壁多糖由4部分组成，分子量分别为15 000、37 000、66 000、150 000。其中最具抗原性的部分是37 000，37 000的多糖部分和牛心瓣膜糖蛋白能同时与风湿性心瓣膜炎的血清发生免疫反应。而病人血清中的抗体被瓣膜糖蛋白吸附后，则不再与细菌多糖反应。结论 A组β溶血性链球菌壁多糖的最具抗原性的部分是37 000，该部分与牛心瓣膜糖蛋白呈现交叉反应性。
【关键词】 多糖类，细菌 心脏瓣膜糖蛋白 抗原性 交叉反应 色谱法，凝胶
Antigenity and cross-reactivity in group A streptococcal polysaccharide
PENG Chaoquan YU Buyun CHEN Guoqing,et al.
(Third Affiliated Hospital,Sun Yat-sen University of Medical Sciences,Guangzhou 510630)
【Abstract】 Objective To study the portion with strongest antigenity of group A β-hemolytic streptococcal polysaccharide (PS),and a cross-reactivity between PS and heart valvular structural glycoprotein (VSGP) of bovine.Methods Molecular sieve chromatography was adopted to extract and purify PS.Double immunodiffusion in agarose was used to distinguish the portion with strongest antigenity of PS,and detect a cross-reaction between this portion of PS and VSGP.Results It was found that PS was composed of four portions,their molecular weights were about 15 000,37 000,66 000 and 150 000 respectively.The portion with strongest antigenity had a molecular weight of 37 000.Both this portion of PS and VSGP could react on the sera of patients with rheumatic valvulitis.After antibodies in the sera were adsorbed by VSGP,no reaction was found between PS and sera.Conclusion The portion with strongest antigenity of PS has a molecular weight of 37 000.A cross-reactivity existed between PS of streptococci and VSGP of bovine.
【Key words】 Polysaccharides,bacterial Heart valvular structural glycoprotein Antigenity Cross reactions Chromatography,gel
风湿热的发病机制尚未
明了，但A组β溶血性链球菌咽喉部感染引发的自身免疫反应的现象已为国际上所公认〔1〕。因此只有深入研究链球菌和宿主的关系，才有可能进一步了解风湿热的发病机制，A组链球菌的细胞结构和细胞外产物均具有致风湿热的抗原性〔2〕，而细胞结构具有特异性意义。国外研究发现，A组链球菌胞壁多糖(PS)与牛
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