High expression of Cullin1 indicates poor prognosis for NSCLC patients(8)

M.Xuetal./Pathology–ResearchandPractice210(2014)397–401

401

paraf n-embeddedslices,whichshowedthatCullin1wascorrela-tivelyexpressedwithKi-67.BothCullin1andKi-67werehighlyexpressedinpoorlydifferentiatedNSCLCslices(Fig.2).Besides,Cullin1wassigni cantlyassociatedwithhistologicaldifferenti-ation(P=0.002),clinicalstage(P=0.010),andKi-67(P=0.021;Table1).MultivariateanalysisusingtheCox’sproportionalhazardsmodelindicatedthatCullin1mightbeanindependentindicatorofNSCLCpatients’prognosis(P=0.033;Table2).SurvivalcurverevealedthathighCullin1expressioncorrelatedwithpoorsurvivalwithstatisticalsigni cance(P<0.001;Fig.3).Thus,our ndingssupportedthenotionthatCullin1couldbeapositiveregulatorandpotentiallyatherapeutictargetofNSCLC.Sofar,thereisbarelyanystudyaboutCullin1inNSCLC;inthisstudywearethe rsttostudythefunctionofCullin1onNSCLC.

Insummary,wefoundthathighCullin1expressionwassigni -cantlyassociatedwithhighhistologicalgradeandKi-67expressionaswellaspoorprognosisinpatientswithNSCLC.However,alargergroupofpatientsneedstobeinvestigatedtocon rmthisconclu-sion.Consequently,Cullin1expressioncouldaffectoverallsurvivalwithtumorprogressionandthusrepresentedapotentialtargetforthetreatmentofNSCLC.Becausecellproliferationhadessen-tialrolesincarcinogenesis,controllingofcancercellproliferationwasimportantforinhibitingcancerprogression.Ourpresentstud-iessupportedanimportantroleforCullin1inpromotingNSCLCprogression,andsuggestedapossiblepathologicalmechanismofNSCLC.AlltheseindicatedthatCullin1mightfunctionasatherapytargetforNSCLC.Thereby,inordertoclarifythemolecularmecha-nismsofCullin1inNSCLCpathogenesis,furtherstudiesareofgreatnecessity.

Paraf n-embeddedtissuesectionswerestainedwithantibodiesforCullin1andKi-67andcounterstainedwithhematoxylin(A–L).BothCullin1andKi-67werehighlyexpressedinlungsquamouscellcarcinomacells(A,B,E,F,I,J)andlungadenocarcinomacells(C,D,G,H,K,L).AccordingtotheintensityofCullin1expression,wedividedthesamplesintohistologicdifferentiationgradeI(A–D),histologicdifferentiationgradeII(E–H),andhistologicdifferentiationgradeIII(I–L).

Kaplan–MeiersurvivalcurvesforlowversushighCullin1expressionon114patientswithNSCLCshowedahighlysigni cantseparationbetweencurves(P<0.001).

Con ictofinterest

Alltheauthorsdeclarenocon ictofinterest.

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