Restriction sites for pGEX-2T

GE Healthcare

Restriction sites for pGEX-2T

The following restriction site table was compiled using DNASIS software for sequence analysis. The enzymes chosen are those which we believe to have been commercially available in June 1992.

The vector pGEX-2T was not tested with each enzyme and therefore the accuracy of the table cannot be guaranteed. Please contact your local GE Healthcare subsidiary if a discrepancy is identi?ed.

Please note that enzymes with nonpalindromic recognition sequences are listed twice. You must combine both entries to obtain the total number of sites for these enzymes.

Locations represent the 5’ end of the recognition sequence for each restriction enzyme.

Enzyme (# sites): Locations

Asu II (1): 654

Ava I (1): 935

Ava II (4): 752, 1779, 2001, 4199

Ava III (0): Avr II (0):

Bal I (1): 463

Bam H I (1): 930

Ban I (6): 2190, 3567, 4286, 4416, 4696, 4708 Ban II (1): 3854

Bbr P I (0):

Bbs I (3): 1153, 3786, 4125

Bbs I (0):

Bbv I (11): 42, 1010, 1107, 1899, 2088, 2690, 3109, 3127, 4337, 4586, 4659

Bbv I (7): 1710, 2404, 2610, 2613, 3696, 4067, 4196

Bcg I (1): 3950

Bcg I (2): 299, 1616

Bcl I (2): 692, 3661

Bfa I (4): 270, 1950, 2285, 2538

Bfr I (0):

Bgl I (2): 2019, 4662

Bgl II (0):

Bpm I (3): 2053, 3953, 4724

Bpm I (1): 3500

Bpu1102 I (0):

Bsa I (1): 2071

Bsa I (0):

Bsa A I (1): 1123

Bsa B I (0):

Bsa H I (5): 34, 1220, 1602, 3603, 4286

Bsa J I (7): 934, 935, 2871, 4282, 4421, 4558, 4848

Bse A I (0):

Bsg I (2): 69, 523

Bsg I (3): 11, 3513, 3713

Bsi E I (5): 1622, 1771, 2694, 3118, 4636

Bsi W I (0):

Bsi Y I (11): 264, 861, 1016, 2547, 2826, 2992, 3010, 3223, 4069, 4313, 4764

Bsl I (11): 264, 861, 1016, 2547, 2826, 2992, 3010, 3223, 4069, 4313, 4764

Bsm I (0):

Bsm I (0):Bsm A I (5): 2072, 3339, 3744, 3870, 4257 Bsm A I (2): 1015, 1302

Bsp1286 I (6): 18, 1471, 1556, 2717, 3627, 3854

Bsp D I (0):

Bsp E I (0):

Bsp H I (3): 1198, 1303, 2311

Bsp M I (0):

Bsp M I (1): 63

Bsp M II (0):

BspW I (26): 102, 866, 1899, 2019, 2407, 2979, 3093, 3158, 3324, 3414, 3510,

3612, 3699, 3836, 3843, 4044, 4051,

4053, 4195, 4289, 4373, 4417, 4632,

4662, 4671, 4697

Bsr I (14): 1493, 1932, 1975, 2093, 2499, 3461, 3563, 3800, 3952,

4262, 4361, 4522, 4548, 4770

Bsr I (6): 1117, 1667, 2615, 2628, 3302, 3501 Bsr F I (2): 2058, 3333

Bss H II (1): 4058

Bst1107 I (0):

Bst B I (1): 654

Bst E II (1): 3828

Bst N I (11): 764, 2871, 2884, 3005, 3371, 3686, 4226, 4283, 4422, 4559, 4686

Bst U I (27): 360, 925, 971, 973, 1076, 1251, 1583, 2076, 2406, 2987, 3246, 3360,

3397, 3447, 3518, 3536, 3552, 3641,

3648, 3796, 3842, 3877, 4059, 4083,

4314, 4316, 4920

Bst X I (3): 3442, 3571, 3694

Bst Y I (8): 914, 930, 1496, 1513, 2281, 2293, 2379, 2390

Bsu36 I (1): 4739

Cfr10 I (2): 2058, 3333

Cla I (0):

Dde I (8): 624, 1216, 1642, 2182, 2348, 2757, 4219, 4740

Dpn I (23): 660, 693, 915, 931, 962, 1461, 1497, 1514, 1772, 1818, 1836, 2177,

2282, 2294, 2372, 2380, 2391, 2466,

3558, 3662, 4035, 4637, 4733

Aat II (1): 1220

Acc I (0):

Acc65 I (0):

Aci I (19): 924, 1299, 1628, 1795, 2439, 2583, 3079, 3100, 3107, 3206, 3359, 3446,

3675, 4146, 4202, 4303, 4313, 4630,

4835

Aci I (38): 225, 549, 1044, 1083, 1093, 1135, 1252, 1398, 1507, 1584, 1749, 1986,

2077, 2448, 2693, 2814, 2833, 2960,

2988, 3150, 3167, 3193, 3247, 3398,

3414, 3423, 3465, 3537, 3601, 3621,

3624, 3852, 3936, 4092, 4230, 4369,

4472, 4706

Afl II (0):

Afl III (3): 496, 3031, 3647

Age I (0):

Aha II (5): 34, 1220, 1602, 3603, 4286

Alu I (25): 54, 178, 482, 631, 809, 1012, 1031, 1789, 1852, 1952, 2473, 2730, 2776,

2866, 3092, 3427, 3707, 4131, 4246,

4339, 4403, 4498, 4607, 4718, 4898

Alw I (7): 914, 930, 1496, 1817, 2281, 2379, 2465

Alw I (4): 926, 1509, 2289, 2386

Alw N I (1): 2617

Apa I (1): 3854

Apa L I (4): 18, 1471, 2717, 3627

Apo I (3): 940, 3212, 3922

Asc I (0):

Ase I (4): 189, 1967, 4331, 4390

Asu I (13): 290, 752, 1163, 1779, 2001, 2018, 2097, 3509, 3854, 3855, 4199, 4223,

4627

28-4801-01

Dpn II (23): 660, 693, 915, 931, 962, 1461, 1497, 1514, 1772, 1818, 1836, 2177,

2282, 2294, 2372, 2380, 2391, 2466,

3558, 3662, 4035, 4637, 4733

Dra I (5): 683, 792, 1563, 2255, 2274

Dra II (2): 289, 1162

Dra III (0):

Drd I (2): 1060, 2923

Dsa I (1): 4848

Dsa V (20): 764, 935, 936, 1016, 1051, 1606, 1957, 2653, 2871, 2884, 3005, 3266,

3371, 3686, 4075, 4226, 4283, 4422,

4559, 4686

Eae I (4): 463, 1750, 4321, 4524

Eag I (0):

Eam1105 I (1): 2138

Ear I (0):

Ear I (5): 338, 1345, 3149, 3266, 4618

Ecl136 II (0):

Eco47 III (0):

Eco57 I (1): 1456

Eco57 I (1): 2504

Eco N I (1): 264

Eco O109 I (2): 289, 1162

Eco R I (1): 940

Eco R II (11): 764, 2871, 2884, 3005, 3371, 3686, 4226, 4283, 4422, 4559, 4686 Eco R V (1): 4095

Esp I (0):

Esp3 I (1): 4256

Esp3 I (1): 1015

Fnu4H I (28): 42, 1010, 1107, 1398, 1627, 1722, 1749, 1899, 2088, 2416, 2622,

2625, 2690, 2833, 2988, 3106, 3109,

3127, 3414, 3537, 3601, 3621, 3708,

4079, 4208, 4337, 4586, 4659

Fok I (7): 767, 1046, 1691, 1978, 2159, 3680, 3689

Fok I (3): 692, 887, 4579

Fsp I (2): 1918, 4656

Hae II (6): 2787, 3157, 3262, 4043, 4286, 4677

Hae III (20): 291, 464, 859, 1164, 1751, 2018, 2098, 2556, 2990, 3008, 3019, 3374,

3509, 3855, 4224, 4322, 4525, 4627,

4744, 4913

Hga I (3): 726, 1602, 4918

Hga I (8): 25, 1067, 2342, 2920, 3594, 3639, 3868, 3874

Hgi A I (5): 18, 1471, 1556, 2717, 3627

Hha I (34): 109, 138, 359, 972, 1075, 1105, 1250, 1582, 1919, 2012, 2405, 2514,

2688, 2788, 2855, 3125, 3158, 3263,

3519, 3553, 3642, 3843, 3876, 4044,

4051, 4058, 4060, 4082, 4287, 4315,

4380, 4657, 4678, 4793

Hinc II (3): 182, 1599, 4151

Hind II (3): 182, 1599, 4151

Hind III (0):

Hin f I (9): 2144, 2661, 3057, 3132, 3275, 4071, 4326, 4517, 4855

Hinp I (34): 109, 138, 359, 972, 1075, 1105, 1250, 1582, 1919, 2012, 2405, 2514, 2688, 2788, 2855, 3125, 3158, 3263, 3519, 3553, 3642, 3843, 3876, 4044, 4051, 4058, 4060, 4082, 4287, 4315, 4380, 4657, 4678, 4793Hpa I (1): 4151

Hpa II (20): 936, 1017, 1051, 1606, 1848,

1958, 2025, 2059, 2463, 2653, 2679,

2826, 3267, 3334, 3726, 3967, 4075,

4444, 4694, 4712

Hph I (12): 369, 438, 690, 981, 990, 1439,

2061, 2288, 3290, 3365, 4232, 4265

Hph I (3): 1417, 1658, 3823

Kas I (1): 4286

Kpn I (0):

Ksp632 I (0):

Ksp632 I (5): 338, 1345, 3149, 3266, 4618

Mae I (4): 270, 1950, 2285, 2538

Mae II (13): 1, 473, 800, 882, 1124, 1221,

1541, 1914, 2330, 3309, 3382, 4540,

4809

Mae III (19): 699, 949, 1026, 1121, 1483,

1671, 1824, 1882, 2213, 2496, 2612,

2675, 3306, 3829, 4545, 4565, 4785,

4811, 4869

Mam I (0):

Mbo I (23): 660, 693, 915, 931, 962, 1461,

1497, 1514, 1772, 1818, 1836, 2177,

2282, 2294, 2372, 2380, 2391, 2466,

3558, 3662, 4035, 4637, 4733

Mbo II (4): 412, 2375, 3628, 4728

Mbo II (13): 330, 342, 657, 1153, 1349, 1458,

1536, 2291, 3153, 3270, 3786, 4125,

4622

Mlu I (1): 3647

Mnl I (7): 1770, 1976, 2854, 3137, 3992,

4618, 4735

Mnl I (11): 903, 968, 998, 1166, 2113, 2194,

2594, 2918, 3499, 4306, 4764

Msc I (1): 463

Mse I (23): 190, 286, 445, 450, 684, 793,

1192, 1564, 1929, 1968, 2203, 2256,

2270, 2275, 2327, 3544, 3667, 3861,

4152, 4332, 4391, 4575, 4884

Msp I (20): 936, 1017, 1051, 1606, 1848,

1958, 2025, 2059, 2463, 2653, 2679,

2826, 3267, 3334, 3726, 3967, 4075,

4444, 4694, 4712

Mun I (0):

Nae I (0):

Nar I (1): 4286

Nci I (9): 935, 936, 1016, 1051, 1606, 1957,

2653, 3266, 4075

Nco I (0):

Nde I (0):

Ngo M I (0):

Nhe I (0):

Nla III (22): 257, 497, 696, 716, 749, 856,

1007, 1112, 1199, 1304, 1697, 1733,

1811, 1821, 2312, 3032, 3256, 3783,

3962, 3983, 4135, 4507

Nla IV (16): 752, 920, 930, 1254, 1844, 2055,

2096, 2190, 2962, 3001, 3567, 3854,

4286, 4416, 4696, 4708

Not I (0):

Nru I (0):

Nsi I (0):

Nsp I (3): 496, 1006, 3031

Nsp B II (8): 1081, 1505, 2446, 2691, 3675,

4245, 4338, 4606

Pac I (0):

Pae R7 I (0):

Pfl M I (1): 3223

Ple I (1): 2656

Ple I (4): 2144, 3132, 3275, 4071

Pme I (0):

Pml I (0):

Ppu M I (0):

Pst I (1): 1897

Pvu I (2): 1771, 4636

Pvu II (3): 4245, 4338, 4606 Rsa I (3): 830,

1661, 3793

Rsr II (0):

Sac I (0):

Sac II (0):

Sal I (0):

Sau I (1): 4739

Sau3A I (23): 660, 693, 915, 931, 962, 1461,

1497, 1514, 1772, 1818, 1836, 2177,

2282, 2294, 2372, 2380, 2391, 2466,

3558, 3662, 4035, 4637, 4733

Sau96 I (13): 290, 752, 1163, 1779, 2001,

2018, 2097, 3509, 3854, 3855, 4199,

4223, 4627

Sca I (2): 829, 1660

Sce I (0):

Sce I (0):

Scr F I (20): 764, 935, 936, 1016, 1051, 1606,

1957, 2653, 2871, 2884, 3005, 3266,

3371, 3686, 4075, 4226, 4283, 4422,

4559, 4686

Sdu I (6): 18, 1471, 1556, 2717, 3627, 3854

Sec I (7): 934, 935, 2871, 4282, 4421, 4558,

4848

Sfa N I (4): 1682, 3185, 3812, 4003

Sfa N I (10): 759, 1038, 1443, 1883, 2935,

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