小鼠_IL-2_Elisa试剂盒(5)

产品详细说明书

ELISAKitfortheQuantitativeAnalysisofMouseIL-2

ThemouseIL-2ELISA(enzyme-linkedimmunosorbentassay)kitisusedfordetectionofmouseIL-2incellculturesupernatants,mouseserumandplasma.THEELISAKITISFORRESEARCHUSEONLY.Pleasereadthisinstructionmanualcarefullyandcheckoutthematerialprovidedbeforeuse,andyoucancontactwithourcompanyifanyquestions.Youcanenterourwebsiteorcallusforotheraim.

Introduction

Interleukin2(IL-2)isapleiotropiccytokineproducedprimarilybyTlymphocytes.ThematuremouseIL-2isa149aminoacid(aa)residueglycoprotein.Thematureproteiniscleavedfromthe169aminoacid(aa)residueprecursor.

IL-2playsakeyroleinpromotingtheclonalexpansionofantigen-specificTcells,naturalkillercellsandB-cellsduringcertainphasesoftheirresponse.Moreover,IL-2modulatestheexpressionofinterferon-γandmajorhistocompatibilityantigens,stimulatesproliferationanddifferentiationofactivatedB-cells,augmentsnaturalkillercellactivityandinhibitsgranulocyte-macrophagecolonyformation.Inaddition,IL-2inducestheproliferationanddifferentiationofoligodendrocytes.

PrinciplesoftheTest

Thekitsisasolidsandwichenzyme-linkedimmunosorbentassayfordetectionofmouseIL-2.Ananti-mouseIL-2monoclonalantibodyhasbeenabsorbedontothewellsofthemicrotiterstripsprovided.Samplesincludingspecimensorstandardswerepipettedintowells.ThemouseIL-2inspecimensorstandardswouldbecapturedbythecoatedantibodyandthefreeotherswereremovedbywashing.ThemouseIL-2biotin-conjugatedantibodywereaddedandbindstomouseIL-2capturedbythefirstantibody,whichformedasandwich.Streptavidin-HRPwouldbeaddedandbindstothebiotinconjugatedantibody,thenfreeStreptavidin-HRPwouldberemovedduringawashstep.Afterthis,subtratesolutionwouldbeaddedandcatalyzedbytheHRP,andacolouredproductisformed.TheintensityofthecoloredproductisusedtocalculateinproportiontotheamountofmouseIL-2intheoriginalspecimen.

Materialsprovidedwiththekits:

reagentAssayBuffer

IL-2Antibody-CoatedWellsStandardDiluentIL-2StandardDetetionAntibodyStreptavidin-HRP

WashBufferConcentrate20×TMBStopSolutionPlateCovers

CompleteInstructionManual

96/48TestKit5ml/3ml12strips/6strips

10ml/5ml2/1vial(s)10ml/5ml10ml/5ml30ml10ml/5ml5ml/3ml3/21

SpecimenCollection

1.Collectingspecimenasfollowing:

A.Theparticulateofthecellculturesupernatantsshouldberemovedbeforeuse.B.Serumwasobtainedfromclotatroomtemperature.C.PleasecollectplasmawithEDTA.

D.Assayimmediatelyorstoresamplesat－20℃.Avoidfree-thawcycles.2.AntisepticandanticoagulantshouldnotappearinSerumsamples.

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