小鼠_IL-2_Elisa试剂盒(7)

3.Add100ulofstandardorsample.CoverwiththePlateCoversprovided.Incubatefor2hoursatroomtemperature,Ifassaytheserumsample,youshouldadd50μlassaybufferwith50μlsampleintothewells,iftheproteinconcentrationishigherthantherangeoftheKit,addthesamequantitysassaybufferwiththesample,thedeficiencyshouldbecomplementedwithsamplediluentto100μlperwell.

4.Fivetimeswashprocesswererepeated.

5.Add100ulofdetetionantibody.CoverwiththePlateCoversprovided.Incubatefor1houratroomtemperature.6.Fivetimeswashprocesswererepeated.

7.Add100ulofStreptavidin-HRP.CoverwiththePlateCoversprovided.Lucifugalincubationfor20minutesatroomtemperature.

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