Cell adhesion and mechanical properties of a flexible scaffold for cardiac tissue engineering

ARTICLEINPRESS

ActaBiomaterialiaxxx(2007)

xxx–xxx

Briefcommunication

Celladhesionandmechanicalpropertiesofa exiblesca oldfor

cardiactissueengineering

L.A.Hidalgo-Bastidaa,J.J.A.Barrya,N.M.Everittb,F.R.A.J.Rosea,L.D.Butterya,

I.P.Hallc,W.C.Claycombd,K.M.Shakeshe a,*

c

DivisionofDrugDeliveryandTissueEngineering,SchoolofPharmacy,UniversityofNottingham,NottinghamNG72RD,UK

Bioengineering,SchoolofMechanical,MaterialsandManufacturingEngineering,UniversityofNottingham,NottinghamNG72RD,UK

DivisionofTherapeuticsandMolecularMedicine,SchoolofMedicalandSurgicalSciences,UniversityHospitalNottingham,NottinghamNG72UH,UK

d

BiochemistryandMolecularBiology,SchoolofMedicineatNewOrleans,LouisianaStateUniversity,BatonRouge,LA,USA

b

a

Received21March2006;receivedinrevisedform7December2006;accepted12December2006

Abstract

Cardiactissueengineeringisfocusedonobtainingfunctionalcardiomyocyteconstructstoprovideanalternativetocellularcardio-myoplasty.Mechanicalstimulihavebeenshowntostimulateproteinexpressionandthedi erentiationofmammaliancellsfromcon-tractiletissues.Ouraimwastoobtaina exiblesca oldwhichcouldbeusedtoapplymechanicalforcesduringtissueregeneration.Poly(1,8-octanediol-co-citricacid)(POC)isanelastomerthatcanbeprocessedintosca oldsfortissueengineering.Weinvestigatedthee ectofmodifyingtheporosityonthemechanicalpropertiesofthePOCsca olds.Inaddition,thee ectsofthestoragemethodandstrainrateonmaterialintegritywereassessed.ThemaximumelongationofPOCporous lmsvariedfrom60%to160%oftheiroriginallength.Adecreaseintheporositycausedariseinthiselasticmodulus.TheattachmentofHL-1cardiomyocytestoPOCwasassessedon lmscoatedwith bronectin,collagenandlaminin.Theseextracellularmatrixproteinspromotedcelladhesioninapro-tein-type-andconcentration-dependentmanner.Therefore,POCsca oldscanbeoptimisedtomeetthemechanicalandbiologicalparametersneededforcardiacculture.Thisporousmaterialhasthepotentialtobeusedforcardiactissueengineeringaswellasforothersofttissueapplications.

Ó2007ActaMaterialiaInc.PublishedbyElsevierLtd.Allrightsreserved.

Keywords:Sca old;Poly(1,8-octanediol-co-citricacid)(POC);Elastomer;Cardiomyocyte;Tissueengineering

1.Introduction

CardiovasculardiseaseshavebecomethemaincauseofdeathnotonlyinWesterncountriesbutalsoinmanydevel-opingnations[1].Currenttherapiesforcardiacfailure(withtheexceptionofcardiactransplantation)ingeneralprovidelimitedimprovementsincardiacoutputandrelatedsymp-toms.Cardiactransplantation,thebesttherapyavailable,isrestrictedbytheshortageofdonorsandtheriskoftissuerejection.Recentresearchhasconcentratedonnewalterna-tives,suchascardiaccellularmyoplasty,whichcould

Correspondingauthor.Tel.:+441159515104.

E-mailaddress:kevin.shakeshe @nottingham.ac.uk(K.M.Shake-

she ).

*potentiallyimproveoutcomesintermsofbothmorbidityandmortality[2].Apotentialalternativetreatmentcouldbeachievedbyusingaphysicalsupportcontaininggrowthfactorscapableofinducingspeci ccellulardi erentiationtogenerateanengineeredcardiacconstruct.Aninitialchal-lengeofgeneratinganengineeredcardiacpatchisthedevel-opmentoftheidealbiomaterialforcardiomyocyteculture.Researchershaveproposedbothsyntheticandnaturalmaterialstogenerateasuitablecardiacgraft.Naturalpoly-merssuchascollagenwere rstusedforcardiacsca olds,buttheyexhibitedlimitedmechanicalpropertiesandbatch-to-batchvariation[3].RadisicandVunjak-Novako-vicstatedthatcollagenisnotacandidateforcardiacscaf-foldsbecauseofits‘‘poorstructuralintegrity’’[4].Syntheticpolymershavealsobeeninvestigated;Bursac

1742-7061/$-seefrontmatterÓ2007ActaMaterialiaInc.PublishedbyElsevierLtd.Allrightsreserved.doi:10.1016/j.actbio.2006.12.006

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