Melatonin anterior pituitary of the female Wistar rat(4)

280

W. Zhaoet al.

Fig. 1.Immunohistochemical stain-ing of female Wistar rat anteriorpituitary gland for expression ofPRL, VEGF, MMP-9, and AQP-1.V-vehicle control rats, DES-diehyl-stilbestrol treated rats, DES+M-diehylstilbestrol treated rats coad-ministrated with melatonin. scalebars=30µm.

group, possibly attributed to the sharp withdrawal ofproliferated cells.

The H+E staining-based evaluation scores in thevehicle, DES, and DES + melatonin groups were1.42±1.13, 4.57±0.98, and 2.00±0.63, respectively,and frequency distribution of values in each groupwere in agreement with normal distribution. Long-term treatment of DES significantly increased the H+Escore, which was reduced by subcutaneous injection ofmelatonin (p<0.01 vs. DES group). In the sole pituitarywith spontaneous tumor-like changes, the tumor-likecells were closely packed with similar cytoplasmic andnuclear volumes, suggesting that they were from thesame origin.

blood vessels. By contrast, administration of DES ledto extensive expression of VEGF in the cytoplasm ofpituitary cells, accompanied by angiogenesis, whichwas reduced by melatonin at a dose of 1 mg/day for 4weeks (Fig. 1).

搜索“diyifanwen.net”或“第一范文网”即可找到本站免费阅读全部范文。收藏本站方便下次阅读，第一范文网，提供最新工程科技Melatonin anterior pituitary of the female Wistar rat(4)全文阅读和word下载服务。