BSA牛血清白蛋白(2)

BSA牛血清白蛋白

Diffusion constant, D20,W × 107 cm2/s: 5.9[5][4] Partial specific volume, V20: 0.733[5][4] Intrinsic viscosity, η: 0.0413[5][4] Frictional ratio, f/f0: 1.30[5][4] Refractive index increment (578 nm) × 10-3: 1.90[5][4] Optical absorbance, A279nm1 g/L: 0.667[5][4] Mean residue rotation, [m']233: 8443[5][4] Mean residue ellipticity: 21.1 [θ]209 nm; 20.1 [θ]222 nm[5][4] Estimated a-helix, %: 54[5][4] Estimated b-form, %: 18[5][4]

BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. It is also used as a nutrient in cell and microbial culture.[citation needed] In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels.[7] This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA (see Bradford protein assay). BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry.

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