Growth and accelerated differentiation of mesenchymal stem cells on graphene oxide(2)

JournalofMaterialsChemistryBPaper

simpleandversatilemethodtoimmobilizefunctionalmole-cules.16–23Herein,theuniquepropertiesofGO,i.e.alargesurfaceareaandexcellentmechanicalproperties,couldbesynergicallycoupledwiththebiocompatibilityofPLLresultinginakindofmultifunctionalsca oldcoating,whichcanbeusedintissueengineeringandstemcell-basedtherapy.

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2Experimental

2.1

Materials

Expandablegraphite($180mm)wasobtainedfromQingdaoBCSM.CO.,LTD.Poly-L-lysine(PLL)andphosphatebu ersolution(PBS)werepurchasedfromSigma-Aldrich(USA).Cal-cellswithgreencolor)andethidiumhomodimer(tostaindeadcellswithredcolor)wasemployed.Forthepurposeofcomparison,cellcountingkit-8(CCK-8)assays(Dojindo,Japan)werecarriedoutforallthestudiedcells.ThemorphologyoftheMSCsonallthesurfaceswasobservedbyusingaphasecontrastmicroscope(OlympusCKX31,Japan).Thepopulationdoublingtimes(PDT)ofcellsonallthestudiedsurfacesweredeterminedwiththegrowthkineticsandthefollowingequation.27

PDT¼ðt2Àt1Þ

lg221

(1)

N2:numberofcellsattimet2,andN1:numberofcellsattimet1.cein-AMandethidiumhomodimerwereobtainedfromDojindo(Japan).Antibodiesincludinganti-CD-44andanti-osteocalcin(OCN)werepurchasedfromAbcam(HongKong).Waterusedinthisworkwaspuri?edbyaMilli-Qwatersystem(Millipore,USA).2.2

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