亚粒径阳离子交换色谱(12)

赛分科技离子交换色谱产品具有多种规格的粒径可供选择,小粒径填料产品对样品分离达到高分辨率、稳定的完美效果。

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10 Separation of BSA Digest on Proteomix ® SCX NP1.7 2.1 x 30 mm

Figure 14. Separation of BSA tryptic digest on Proteomix ® SCX NP1.7 2.1 x 30 mm. Mobile phase A: 10 mM phosphate buffer pH 2.5, B: A + 1 M NaCl and C: 100% acetonitrile. Flow rate was 0.35 mL/min. UV detection was set at 214 nm. 5 and 10 µg of trypsin digested BSA was injected on the column.

Separation of Peptides on Proteomix ® SCX NP1.7 2.1 x 30 mm

Figure 15. Separation of four Peptides on Proteomix ® SCX NP1.7 2.1 x 30 mm. Mobile phase A: 10 mM phosphate buffer pH 2.5, B: A + 1 M NaCl and C: 100% acetonitrile. Flow rate was 0.35 mL/min. UV detection was set at 214 nm. 2 µL of the peptide

mixture was injected.

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