猪戊型肝炎(6)

3. RNA extracted and a RT-nPCR was performed using primers designed based on the HEV open reading from 2 (ORF2) sequences. The RT-nPCR products were then sequenced. The resulting nucleotide sequences were compared with the representative strains of HEV genotypes I-IV and a phylogenetic tree was constructed. Two nucleotide sequences were detected from the 114 HEV positive liver samples and were used for nucleotide sequence analysis. Sequence comparison using the DNAMAN and MEGA4.0 softwares showed that the two sequences shared a homogeneity of 99.2% and shared 74.9%~78.7%, 73.9%~73.9%，74.6%~77% and 81.2%~93.7% homogeneity with HEV genotypes Ⅰ,Ⅱ,Ⅲ, and Ⅳ, respectively. Phylogenetic tree analyses revealed that the two sequences detected were closely related to HEV 87, 277 and 292 strains and were grouped into genotype Ⅳ.

4. The purpose of the present study was to determine the prevalence of swine hepatitis E virus（HEV）infection in the slaughtered swine. One hundred and five swine serum samples collected from Beijing several butchery were tested by ELISA for the presence of total antibodies against HEV. The result showed that anti-HEV total antibody were detected in 93 out of 105 pigs. The seropositivity rate was 88.57%. It suggested that slaughtered swine HEV was widespread.

5. One hundred and thirty nine slaughtered swine serum samples collected from Beijing several butchery were tested for the level of ALT and AST in serum. The result showed that the abnormality rate of ALT was 76.98%、the abnormality rate of AST was 79.10% and the simultaneous abnormality rate of ALT and AST was 69.40%.

搜索“diyifanwen.net”或“第一范文网”即可找到本站免费阅读全部范文。收藏本站方便下次阅读，第一范文网，提供最新教学研究猪戊型肝炎(6)全文阅读和word下载服务。