体外微核试验方法研究进展(4)

体外微核试验方法研究进展

及一个γ卫星DNA探针信号者,则由落后的染色单体形成(25)。

体外微核试验有简便、经济、快速和灵敏度较高等特点。随着研究的深入和新方法的不断发展完善,可望成为短期筛测的主要手段。参考文献

1　FenechM,andMorleyA.Measurementofmicronucleiinlympho2cytes.MutatRes,1985;147:29

2　FenechM.Thecytokinesis2blockmicronucleusthchnique:Adetailed

descriptionofthemethodanditsapplicationtogenotoxicitystudiesinhumanpopulations.MutatRes,1993;285:35

3　LeeTK,JohnsonJ,WileyAL,etal.Assessmentoftwoprotocolsforthehumanlymphocytecytokinesis2blockedmicronucleusassay.

Mutagenesis,1995;10:375

formationbycoal2dustextractinBALB/3T3A31-1-13cellline.

MutatRes,1990;242:225

13　GuZW,WhongWZ,WallaceWE,etal.Inductionofmicronuclei

inBABL/c-3T3cellsbyselectedchemicalsandcomplexmixture.

MutatRes,1992;279:217

14　吴健敏,余应年,陈星若1转基因细胞CHL-2B6的建立和在遗

传毒理试验代谢活化中的应用1中国公共卫生学报,1996;15

(2):111

15　蔡朱男,董海涛,余应年1转染了细胞色素P4501A1基因重组表

达质料的CHL细胞(CHL-1A1)可化谢活化多环芳烃类化合物1癌变 畸变 突变,1996;8(3):136

16　KormosC,andKotelesGJ.MicronucleiinX2irradiatedhumanlym2

phocytes.MutatRes,1988;199:31

17　PartonJW,GarriottML,andBeyersJE.Expulsionodemecolcine2

inducedmicronucleifrommousebonemarrowpolychromaticerythro2cytes.EnvironMolMutagen,1991;17(2):79

18　LidholmC,NorppaH,HayashiM,etal.Inductionofmicronuclei

andanaphaseaberrationsbyinhumanlymphocytecul2tures.MRes,;26019N,al.Evaluationofthemi2

ahamstercelllineasanalternativetoinvitrochromosomalaberrationtest.MutatRes,;272:223

20　CaoJ,etal.Flowcytometricanalysisofinvitromicronucleusinduc2

tioninhepatocytestreatedwithcrainogens.Toxicinvitro,1993;7(4):447

21　KamiguchiY,TatenoH,andMikamoK.Micronucleustestin2-cellembryosasasimpleassaysystemforhumanspermchromosomeaberrations.MutatRes,1991;252:297

22　陈壁锋,黄天华,李来玉,等1人精子2细胞胚微核技术在化学诱

4　顾祖维,钟宝珍1体外细胞微核试验的制片新技术介绍1卫生毒

理学杂志,1991;5(2):108

5　HayashiM,SofuniT,andIshidateMJr.Anapplicationofacridineorangefluoresentstainingtothemicronucleustest.1983;120:241

6　FenechM.Theoriginofmicronucleiinducedbycytosineanditssynergisticinteractionwithhydroxyurea2cytes.Mutagenesis,1994;9:273

7　SurrallesJ,XamenaNAmicronucle2usassayinhumanasaofexcisionrepair.

MutatRes,1995;342MutatRes,

8　ZhangLS,HonmaM,HayshiM,etal.AcomparativestudyofTK6

humanlymphoblastoidandL5178Ymouselymphomacelllinesintheinvitromicronucleustest.MutatRes,1995;347:105

9　AmundsonSA,andChenDJ.Inversedose2rateeffectformutationin2

ductionbygamma2raysinhumanlymphoblasts.IntJRadiatBiol,1996;69(5):555

10　XiaF,WangX,WangYH,etal.AlteredP53statuscorrelates

withdifferencesinsensitivitytoradiation2inducedmutationandapoptosisintwocloselyrelatedhumanlymphoblastlines.Cancer

Res,1995;55(1):12

变研究中的应用1癌变 畸变 突变,1996;8(4):211

23　BakkerPJ,TukkerLJ,StapJ,etal.Micronucleiexpressionintu2

morsasatestforradiationsensitivity.RadiotherOncol,1993;26:69

24　LynchAM,andParryJM.Thecytochalasin2Bmicronucleus/kineto2

choreassayinvitro:studieswith10suspectedaneugens.Mutat

Res,1993;287:71

11　SchechtmanLM.Metabolicactivationofprocarcinogensbysubcellu2

larexzymefractionsinc3H10T1/2andBALB/c-3T3celltransfor2mationsystems.IARCSciPubl,1985;67:137

12　WuZL,ChenJK,OngT,etal.Inductionofmorphologicaltrans2

25　MillerBT,andNusseM.Analysisofmicronucleiinducedby2-chlorobenzylidenemalonitrile(CS)usingflorescenceinsituhybridiza2tionwithtelomericandcentromericDNAprobes,andflowcytome2try.Mutagenesis,1993;8(1):35

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