chosethescale-upcriteriafactorsinfluencingthefermentationprocessandthus
andacetateOilandmethodproped5theeffectsofcarbondioxide
CarbondioxideplaysaHLCproductionwasstudiedfirstly.majorroleinbothaerobicandanaerobicfermentations.However,
culturefewliteraturesfocusedon氇eeffectsofcarbon
targetproteindioxi如onrecombinant互coliandproduction.Inthispaper,asystematicstudywasinitiallycarriedoutwiththe
methodtoinvestigatetheinfluenceofcarbondioxide
C02oncarbondioxidepulsecultivationsystemofinjecfiontherecombinant置coli.mpulse蠲ectionexperimentsshowedthat:
l_)a20%C02pulseintroducedinthebatchcultivationphasesinhibitedcellgrowthobviouslywhilethatintroducedinthefed-batchcultivationphasesstimulatedcellgrowth
inhibitoryeffectofC02onslightly;and2)HLCexpressionoccurredonlyintheexpressionphase,wherethe
by33。9%underafinalHLCconcentrationdecreased3-h20%C02pulse.Meanwhile,the
highertheC02concentrationand/orthelongerthedurationoftheC02pulses,thestrongerthestimulatoryorinhibitoryeffects.However,theC02concentrationbelow
hadlittleinfluenceonorequalto10%theHLCproduction.
Then,asystematicstudywascardedouttodeterminetheeffectsofacetateonthehighcelldensitycultureofrecombinantEcolicontainingHLCmRNA.Inthisstudy,theeffectsofacetateassimilationthrougha番ucose
astarvationperiodatdifferentcellgrowthphaseswereinitiallyinvestigatedinfed-batchcultureofrecombinantEcoli。Theresultsshowedthat
onacetateassimilationwasobservedwithoutnegativeeffects
starvationperiodtheprocessduringaglucoseintroducedatalldefinedcellgrowthphasesexceptforthepost-inductionphase。Inaddition,anewmethodforevaluatingtheeffectsofacetateWasdevelopmentbasedontheabovefindings:theacetateconcentrationwascontrolledbyaddingacetateintoculture
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