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重组E.coli生产类人胶原蛋白发酵调控策略与500L中试规模放大方法优化(5)

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chosethescale-upcriteriafactorsinfluencingthefermentationprocessandthus

andacetateOilandmethodproped5theeffectsofcarbondioxide

CarbondioxideplaysaHLCproductionwasstudiedfirstly.majorroleinbothaerobicandanaerobicfermentations.However,

culturefewliteraturesfocusedon氇eeffectsofcarbon

targetproteindioxi如onrecombinant互coliandproduction.Inthispaper,asystematicstudywasinitiallycarriedoutwiththe

methodtoinvestigatetheinfluenceofcarbondioxide

C02oncarbondioxidepulsecultivationsystemofinjecfiontherecombinant置coli.mpulse蠲ectionexperimentsshowedthat:

l_)a20%C02pulseintroducedinthebatchcultivationphasesinhibitedcellgrowthobviouslywhilethatintroducedinthefed-batchcultivationphasesstimulatedcellgrowth

inhibitoryeffectofC02onslightly;and2)HLCexpressionoccurredonlyintheexpressionphase,wherethe

by33。9%underafinalHLCconcentrationdecreased3-h20%C02pulse.Meanwhile,the

highertheC02concentrationand/orthelongerthedurationoftheC02pulses,thestrongerthestimulatoryorinhibitoryeffects.However,theC02concentrationbelow

hadlittleinfluenceonorequalto10%theHLCproduction.

Then,asystematicstudywascardedouttodeterminetheeffectsofacetateonthehighcelldensitycultureofrecombinantEcolicontainingHLCmRNA.Inthisstudy,theeffectsofacetateassimilationthrougha番ucose

astarvationperiodatdifferentcellgrowthphaseswereinitiallyinvestigatedinfed-batchcultureofrecombinantEcoli。Theresultsshowedthat

onacetateassimilationwasobservedwithoutnegativeeffects

starvationperiodtheprocessduringaglucoseintroducedatalldefinedcellgrowthphasesexceptforthepost-inductionphase。Inaddition,anewmethodforevaluatingtheeffectsofacetateWasdevelopmentbasedontheabovefindings:theacetateconcentrationwascontrolledbyaddingacetateintoculture

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