largerscaleforthe
areathat,1)theoperationparameterscouldbedirectlytransferredtothemembranehomogenization;2)the
proportionallyforoperationparametersrelatedtothewere,increasedwasscaling—UPtheultrafiltrationprocess;3)theprecipitationtimeoptimized
thanthatonthepilot-scaleandtheoptimizedprecipitationw.as70min,whichislittlehigherthelab-scale;4)CM52chromatographyscaling—upbasedononconstantlinearvelocitywassuccessful.ThepurityandrecoveryofHLCreached95.4%,65.4%,repectively,whichweredecreasedslightlycomparedtothose(theputityandrecoverywere96.4%,71.7%,respectively)obtainedonthelab—scale.
Keywords
RecombinantEcoli,human—likecollagen,C02pulse,acetate,oxygentransfer,scale-up
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