Finally,thescale—upfermentationsbasedOilkLaandp*kLawerecarriedoutrespectively,accordingtothecorrelationsforkLacalculation.BecausetherecombinantsystemwaslesssensitivitytotheC02,pressurizedculturewassuccessfulfortheHLCproduction.Thatis,forthenon.inducedcultivation
thefinalonthepilot—scalebasedontheconstantp*kLascale-upcriteria,onDCW
andwas77.9g/L,whichWassimilartolowerthanthethat(80.3g/L)obtainedthelab-scaleoncultivationvalue(90.0g/L)obtainedinthepilot-scalebasedthe
constantkLascale-upcriteria.However,theinitialmediumvolumefortheformer(285L)wasmuchhigherthanthelatter(105L).ThefinalDCWandHLCconcentrationwere68.4g/Land13.0g/Lrepectively,whichweresimilartothoseobtainedonthelab-scale,fortheinducedcultivationonthepilot—scale.
onTheprocess
majorforHLCpurificationunitsthepilot-scalewasalsodevelopedinthisrecoveryandstudy.TheoperationinfluencingHLCpuritywerehomogenization,precipitation,ultrafiltrationandCM52chromatography.Thescale—upinvestigationshowed
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